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Pharmacokinetics, Metabolism and Disposition of [14C]XQ-1H After Intravenous Administration to Male Rats

[ Vol. 10 , Issue. 4 ]

Author(s):

Yinlin Qin, Tao Chen, Qiu Jin, Kai Guo*, Hao Feng, Dennis Heller and Zhe-ming Gu   Pages 228 - 239 ( 12 )

Abstract:


Objective: This study describes the in vivo pharmacokinetics and metabolism of [14C]labeled XQ-1H in male rats.

Methods: XQ-1H is a methanesulfonate of XQ, 10-O-(N,N-dimethylaminoethyl)-ginkgolide B, a derivative of ginkgolide B (GB) with enhanced water solubility. Since it is very difficult to synthesize radiolabeled GB, the results obtained in this study may provide helpful insight to further ADME investigation of GB and its analogue compounds. After an i.v. administration of [14C]XQ-1H to male rats, XQ (the freebase form of XQ-1H) was extensively hydrolyzed, moderately metabolized, and mainly excreted in feces (71.5% of the dose) via the biliary route.

Results: The main enzyme mediated metabolic pathways were mono- and di-demthylation. Using the radiolabel form of XQ-1H, the temporal binding of XQ to red blood cells was observed.

Conclusion: Binding of XQ to RBCs may lower the blood’s viscosity and thus provide symptomatic improvement of ischemic stroke patients.

Keywords:

[14C]XQ-1H, ginkgolide B derivative, high resolution LC-MS, ischemic stroke, mass balance, metabolism, PAF antagonist, radiolabeling technology.

Affiliation:

College of Biotechnology and Pharmaceutical Engineering, Nanjing Technology University, Nanjing 210009, Jiangsu, Jiangsu Carephar Pharmaceutical Co., Ltd, Nanjing 210014, Jiangsu, Jiangsu Carephar Pharmaceutical Co., Ltd, Nanjing 210014, Jiangsu, College of Biotechnology and Pharmaceutical Engineering, Nanjing Technology University, Nanjing 210009, Jiangsu, XBLChina, Inc., a Subsidiary of LTD, WuXi AppTec, Nanjing, Jiangsu 210038, XenoBiotic Laboratories, Inc., a Subsidiary of LTD, WuXi AppTec, Plainsboro, New Jersey, 08536, XBLChina, Inc., a Subsidiary of LTD, WuXi AppTec, Nanjing, Jiangsu 210038

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